You are currently viewing an outdated version of
SubtiWiki.
Please use
the newest version!
trmR [2019-07-01 09:26:04]
tRNA wobble uridine modifying methyltransferase
function
introduction of 5-methoxyuridine modification in tRNA
product
tRNA wobble uridine modifying methyltransferase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]Gene
Coordinates
2,795,082 → 2,795,735
The protein
Catalyzed reaction/ biological activity
methylation of (5-hydroxyuridine) ho5U containing tRNA to 5-methoxyuridine (mo5U) [pubmed|29982645]Protein family
[SW|Methyltransferase superfamily] (according to UniProt)[SW|Cofactors]
SAM [pubmed|29982645]Structure
[PDB|5ZW3] [pubmed|29982645][PDB|5ZW4] (bound to tRNA) [pubmed|29982645]Expression and Regulation
Operons
genes
[gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]-[gene|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN]-[gene|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|yrrO]-[gene|A659D9894E777D8A99707CB750DF2918AEE06CE9|udk]-[gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A853 (yrrM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/853 NBRP B. subtilis, Japan]BKE27360 (Δ[gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTACAAACAGCCTCCCGT, downstream forward: _UP4_AGTAAAAAGAAGAGGTGAACBKK27360 (Δ[gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTACAAACAGCCTCCCGT, downstream forward: _UP4_AGTAAAAAGAAGAGGTGAACReferences
